Optimization of red pigment production by monascus purpureus FTC 5356 using response surface methodology

Optimization of red pigment production by monascus purpureus FTC 5356 using response surface methodology. IIUM Engineering Journal, 19 (1). pp. 34-47. ISSN 1511-788X (2018)



Abstract

Factors such as environmental conditions and nutrients are significant for successful growth and reproduction of microorganisms. Manipulations of the factors are the most effective way to stimulate the growth of the microorganism, which can be used to optimize the yield of a product. In this study, Central Composite Design (CCD) of Response Surface Methodology (RSM) was used to optimize the production of red pigment by Monascus purpureus FTC 5356 using the petioles of oil palm fronds (OPF) as a substrate in solid state fermentation (SSF). The data was analyzed using Design Expert Software. The optimum combination predicted via RSM was confirmed through experimental work. The interactions between three variables such as initial moisture content (%), initial pH value (pH), and peptone concentration (%) were studied and modelled. The statistical analysis of the results showed that the optimal conditions for red pigment production 47 AU/g with the biomass of 425.1 mg/g was at 55% initial moisture content, 3% of peptone, and at pH 3. The RSM results showed that the initial pH value had a significant effect on red pigment production (P-value <0.05). The validation of these results was also conducted by fermentation with predicted conditions and it was found that there was a discrepancy of 0.39% between the values of the experimental result and those of the predicted values.

Item Type: Article
Keywords: Response surface methodology, Red pigment, Oil palm frond, Monascus pigment
Taxonomy: By Subject > College of Engineering > Chemical Engineering > Biotechnology & Bioprocess
Local Content Hub: Subjects > College of Engineering
Depositing User: Eza Eliana Abdul Wahid
Date Deposited: 14 Dec 2022 07:19
Last Modified: 14 Dec 2022 07:19
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