Role of substrate binding on the protein dynamics of an endoglucanase from fusarium oxysporum at different temperatures. IIUM Engineering Journal, 19 (1). pp. 307-314. ISSN 1511-788X (2018)
Abstract
Thermo stability is an important requirement for protein function, and one goal of protein engineering is improvement of activity of the enzymes at higher temperatures, particularly for industrial applications. Computational approaches to investigate factors influencing thermo stability of proteins are becoming researchers’ choice. This study investigates the influence of substrate binding on the protein dynamics by comparing the molecular dynamics simulations of substrate-enzyme complex against un-bound enzyme, using endoglucanase I from Fusarium oxysporum. Endoglucanase-substrate complex was prepared by docking and molecular dynamics simulations were carried out at three different temperatures, 313 K, 333 K and 353 K. Our finding shows that the secondary structures for substrate-enzyme complex show more fluctuations relative to un-complexed structure. The same trend was observed for solvent accessible surface area and radius of gyration. At the highest temperature studied (353 K), the substrate-enzyme complex form showed the highest fluctuations. The fluctuations around the active site regions reach a minimum at the optimum temperature, compared to the other structural regions and other temperatures.
| Item Type: | Article |
|---|---|
| Keywords: | Thermostable enzymes, Thermostability factors, Ligand-enzyme complex, Fusarium oxysporumendoglucanase, Molecular dynamicssimulations |
| Taxonomy: | By Subject > College of Engineering > Chemical Engineering > Biotechnology & Bioprocess By Subject > College of Engineering > Chemical Engineering > Chemical Processes |
| Local Content Hub: | Subjects > College of Engineering |
| Depositing User: | Eza Eliana Abdul Wahid |
| Date Deposited: | 15 Dec 2022 06:15 |
| Last Modified: | 15 Dec 2022 06:15 |
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