Phylogenetic analysis and comparative genomics of brucella melitensis in human brucellosis: a review. Science Letters, 17 (1). pp. 105-120. ISSN 2682-8626 (2023)
Abstract
Brucellosis is a significant zoonotic disease caused by certain species of Brucella and has a wide range of clinical severity in humans. Brucella melitensis is the most virulent human species associated with acute and chronic Brucella disease. The genome of B. melitensis is remarkably conserved, with less than 3% variations between strains from diverse geographical locations. Hence, whole genome sequencing (WGS) analysis has provided broad information on genomic variations of this species and to understand the epidemiology and geographical origin among different strains. This review paper discussed the availability of WGS data could allow for the screening of multiple genomes, identify the SNP variations and detect the emergence of novel virulence genes that could be associated with pathogenicity mechanisms among B. melitensis. The phylogenomic analysis allows the clustering of known strains into five clades, which corresponds well with clinical and geographical features. Recent methods such as core genome multi-locus sequence typing (cgMLS) and multi-locus variable-number tandem-repeat analysis (MLVA) have allowed the study of large epidemiological datasets with high resolution. As Brucella genomes are highly conserved at the species, comparative genomic analysis has provided high-quality information and allowed for high-resolution analysis of the phylogenomic relationship of the species. The current review presents an overview of highlights emergence of novel virulence genes and the evolutionary path of B. melitensis in phylogenomic analysis, which allow us to conduct trace-back studies and correlate genotypes with the geographical origin of the strains.
| Item Type: | Article |
|---|---|
| Keywords: | Brucellosis, Brucella melitensis, Whole genome sequencing, Phylogenetic, Multiplelocus sequence typing |
| Taxonomy: | By Niche > Genome > Bacterial Genomes By Niche > Genome > Genomes By Niche > Genome > Microbial Genomes |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 09 Mar 2023 09:15 |
| Last Modified: | 09 Mar 2023 09:15 |
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