Standardised extracts of moringa oleifera and centella asiatica enhanced the antioxidant activity, learning and memory effects by inhibiting acetylcholinesterase activity in D-Galactose induced ageing rats. Pertanika Journal of Science & Technology, 28 (1). pp. 293-310. ISSN 2231-8526 (2020)
Abstract
The potential of Moringa oleifera Lam. (Moringaceae) and Centella asiatica (L.) Urban (Apiaceae) extracts (TGT-PRIMAAGE) in slowing the decline of memory and learning activity was investigated using D-galactose-induced ageing rat model. The extracts were profiled and standardised based on markers identified using LC/MS-QTOF. Toxicity study of the extract was done, and the rat did not show any sign of toxicity. The extract was orally administered to the rat and dose dependent (100, 500 and 1000 mg/kg) efficacy were investigated. The rats were subjected to Morris Water Maze whereby 3 parameters were studied (number of entry to platform, latency and novel object recognition). Plasma was collected for the determination of catalase (CAT) activity and levels of malondialdehyde (MDA) and advanced glycation end products (AGEs). The activity of acetylcholinesterase (AChE), level of acetylcholine (ACh) and lipid peroxidation (LPO) were measured using the brain lysates. Significant improvement (p < 0.05) was seen in the memory and learning abilities in the aged rats that received medium and high dose of TGT-PRIMAAGE, and tocotrienol. Rats treated with TGT-PRIMAAGE had also shown improved CAT activity and resulted in reduced LPO. The level of ACh was found increased in parallel with the reduced AChE activity. The capabilities of learning and memory of the TGT-PRIMAAGE treated rats were enhanced via inhibition of AChE activity and subsequently increased level of ACh.
| Item Type: | Article |
|---|---|
| Keywords: | Anti-ageing, Antioxidant, Centella asiatica, D-galactose, Moringa oleifera |
| Taxonomy: | By Niche > Genome > Genomes By Niche > Genome > Plant Genomes |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 04 Sep 2023 09:11 |
| Last Modified: | 04 Sep 2023 09:11 |
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