Comparative evaluation of three different methods of genomic DNA extraction for Staphylococcus aureus. World Applied Sciences Journal, 21 (3). pp. 424-427. ISSN 1991-6426 (2013)
Abstract
Extraction of DNA from Staphylococcus aureus is often difficult and less efficient. In this study, the relative recovery of extraction of staphylococcal DNA from pure culture was compared using rapid lysis, bashing beads and commercial silica column methods. For both methicillin - resistant and sensitive Staphylococcus aureus the DNA extracted by rapid lysis method was found to have higher value of DNA concentration than bashing beads and silica column methods. The concentrations of DNA obtained from both methicillin – resistant and sensitive Staphylococcus aureus by rapid lysis method were 74 and 98 µg/ml, while DNA obtained by bashing beads method were 35 and 31 µg/ml in that order from the same strains. The DNA concentrations obtained from the commercial silica column method were 5 and 3 µg/ml correspondingly from the same strains. The present study indicated that rapid lysis method might be a suitable choice for extracting DNA from Staphylococcus aureus strains. For commercial silica column method, high molecular weight intact DNA bands were not observed. Therefore the use of specific and optimized DNA extraction methods is important for the successful PCR on clinical samples.
| Item Type: | Article |
|---|---|
| Keywords: | DNA extraction, Genomic, Staphylococcus aureus, Methicillin-resistant |
| Taxonomy: | By Niche > Genome > Bacterial Genomes By Niche > Genome > Genomes By Niche > Genome > Microbial Genomes |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 29 Sep 2023 09:10 |
| Last Modified: | 29 Sep 2023 09:10 |
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