Development of PCR-based genotyping method for Fat Mass Obesity-Associated (FTO) gene polymorphism RS9939609. Journal of Health and Translational Medicine (JUMMEC), 2 (SI). pp. 51-54. ISSN 2289-392X (2023)
Abstract
Malaysia has the highest obesity rate in Southeast Asia and is growing. Environmental and genetic factors influence weight increase and obesity, leading to numerous diseases and mortality. This study aimed to develop a genotyping method to detect FTO gene variant rs9939609, known for its association with body mass index (BMI) and obesity. In-house primers detected rs9939609. Two common primers flanked the variation region, and two more primers identified the wild type and alternative allele. Capillary sequencing verified results. We developed FTO gene variant rs9939609 genotyping. This method can be used to genotype FTO variant rs9939609 in any research.
| Item Type: | Article |
|---|---|
| Keywords: | Obesity, Body Mass Index (BMI), FTO gene variant rs9939609, Capillary sequencing |
| Taxonomy: | By Niche > Genome > Human Genome |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 06 Feb 2024 09:33 |
| Last Modified: | 06 Feb 2024 09:33 |
| Related URLs: |
Actions (login required)
 |
View Item |