Carriage patterns and susceptibility testing of staphylococcus aureus in healthy nasal carriers in UiTM. Biohealth Science Bulletin 2011, 3 (1). pp. 29-36. ISSN 2550-147X (2011)
Abstract
Staphylococcus is a group of gram-positive bacteria that can cause illness ranging from mild to a number of severe infectious diseases. These bacterial species of the genus Staphyloccus are normal inhabitants of the nostrils in the healthy population with carrier rates between 20% to 55%. This study aims to classify these adults into persistent, intermittent or noncarriers, to differentiate Staphyloccoccus aureus (S. aureus) from other Gram positive cocci and also to obtain the antibiotic sensitivity patterns in this population. From 422 nasal swabs collected, 66 (16%) were S. aureus carriers. Thirty (7%) were persistent carriers (index >0.8-1.0), 36 (9%) were intermittent carriers (index between 0.1-0.7) and 356 (84%) were non-carries (index 0). Only 202 samples were further examined for species differentiation. A total of 37 (18.3%) were identified as S. aureus, 156 (77.2%) S. epidermidis, 8(4%) S. saprophyticus and only 1(0.5%) methicillin resistant S. aureus. From these 202 samples, 13/38 (34.2%) were susceptible to all the 12 antibiotics tested, and 20/38 (52.6%) were resistant to penicillin alone. Tetracycline resistance was 13.2% and low percentages of this S. aureus were resistant to erythromycin (2.6%) and gentamycin (2.6%). Apart from the phenotypic methods, further reconfirmation was performed by the amplification of nuc gene. It is recommended that both molecular and phenotypic analysis for S. aureus can be used to achieve a rapid and accurate identification. In conclusion, this study found that some of the students are intermittent carriers or persistent carriers while majority are noncarriers with no serious threat to methicillin or vancomycin resistance.
| Item Type: | Article |
|---|---|
| Keywords: | Staphylococcus aureus, Nasal carriers rates, Phenotypic analysis, Molecular diagnosis, Bacteria genomic DNA, Antibiotic sensitivity |
| Taxonomy: | By Niche > Genome > Bacterial Genomes By Niche > Genome > Microbial Genomes |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 06 Feb 2024 09:37 |
| Last Modified: | 06 Feb 2024 09:37 |
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