Clinical performance of reverse transcription loop-mediated isothermal amplification COVID-19 assay on gold-nanoparticle-modified screen-printed carbon electrode using differential pulse voltammetry. Sensors and Materials, 35 (10). pp. 4731-4750. ISSN 2435-0869 (2023)
Abstract
The World Health Organization (WHO) has recommended real-time reverse transcription polymerase chain reaction (RT-PCR) as the gold standard for coronavirus disease detection. In this study, we aim to validate the clinical performance of reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay on a gold-nanoparticle-modified screen-printed carbon electrode (AuNP/SPCE) using differential pulse voltammetry (DPV) and to compare it with real-time RT-PCR. The electrodeposited AuNP on SPCE was quasi-spherical with a size of ±500 nm. The developed RT-LAMP primer was designed from the GenBank database using the NCBI Multiple Alignment tools and Jalview software. Nasopharyngeal clinical samples were obtained from suspected COVID-19 patients (n = 148). The RT-LAMP products were dropped on the modified AuNP/SPCE under DPV setting, which resulted in current change (∆I) responses. The positive and negative samples produced significantly different ∆I signals with a p-value <0.0001 at a 95% confidence interval using Student’s t-test. The RT-LAMP assay using Au/SPCE exhibited a 30 s response time per analysis. The clinical sensitivity and specificity obtained were 79.7 and 85.1%, respectively, with a detection limit of 0.4 copies µl−1. Hence, this proposed method is suitable for COVID-19 RNA detection in resource-limited settings.
| Item Type: | Article |
|---|---|
| Keywords: | COVID-19, RT-PCR, RT-LAMP, AuNP/SPCE, differential pulse voltammetry, electrochemical sensor |
| Taxonomy: | By Niche > Genome > Genomes Data Processing By Niche > Genome > Viral Genomes |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 11 Mar 2024 07:26 |
| Last Modified: | 11 Mar 2024 07:26 |
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