Analysis of recurrent parent genome recovery in MR264 X Pongsu Seribu 2 using marker-assisted backcrossing. Malaysian Journal of Genetics (MJG), 1 (12). ISSN 2811-3594 (2021)
Abstract
Marker–assisted backcross (MABC) is a commonly breeding technique used for introgression a blast resistance gene into a rice cultivar. It is considered as an advanced method to overcome the drawbacks of conventional backcross technique and fasten the recovery of recurrent parent genome (RPG). The MABC technique was implemented to produce improve blast resistant rice variety by introgress a blast resistant gene(s) from a traditional variety possess resistant genes (donor), Pongsu Seribu 2 into a local susceptible variety, MR264. The estimation of recurrent parent genome recovery in earlier generation of backcrossing was analysed using simple sequence repeat (SSR) markers. Parental polymorphism analysis with 375 SSR markers showed that only 70 of them were found to be polymorphic. In BC1F1 and BC2F1 generation, background analysis revealed 76.1 to 87.9% and 86.5 to 95.2% of recurrent parent genome recovery, respectively. In selected BC2F2 lines, the average proportion of recurrent genome recovery was 94.4% which showed a phenotypic similarity to MR264. In this study, seven improved lines associated with blast resistance genes and maximum genetic backgrounds of MR264 were identified as improved blast resistant variety. Findings in this study proved the efficiency of MABC technique for rapid recovery of a parental genome in a 2-3 generation of backcrossing population.
| Item Type: | Article |
|---|---|
| Keywords: | Blast, Background selection, Marker-assisted backcrossing breeding, SSR marker, Pongsu Seribu 2 |
| Taxonomy: | By Niche > Genome > Agricultural Genome Mapping By Niche > Genome > Plant Genome Mapping |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 27 May 2024 08:12 |
| Last Modified: | 27 May 2024 08:12 |
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