Coupling genotyping and computational modeling in prediction of anti-epileptic drugs that cause Stevens Johnson Syndrome and toxic epidermal necrolysis for carrier of HLA-B*15:02. The Journal of Pharmacy & Pharmaceutical Sciences, 19 (1). pp. 147-160. ISSN 1482-1826 (2016)
Abstract
The importance of HLA-B*15:02 genotyping to avoid carbamazepine induced SJS/TEN and molecular modeling to predict the role of HLA-B*15:0 and AEDs induced SJS/TEN are investigated. Methods. DNA was extracted from eighty-six patients. The patients were genotyped by AS-PCR. Computational modeling of the HLA-B*15:02 followed by docking studies were performed to screen 26 AEDs that may induce ADR among HLA-B*15:02 carriers. Results. Odd ratio for CBZ induced SJS/TEN and HLA-B*15:02 was 609.0 (95% CI: 23-15873; p=0.0002). Molecular modeling studies showed that acetazolamide, ethosuxiamide, lamotrigine, oxcarbazepine, phenobarbital, phenytoin, primidone and sodium-valproate may induce ADR in HLA-B*15:02 carriers alike CBZ. Conclusion. We confirmed HLA-B*15:02 as a predictor of SJS/TEN and recommend pre-screening. Computational prediction of DIHR is useful in personalized medicine.
| Item Type: | Article |
|---|---|
| Keywords: | Genotyping, HLA-B*15:02, Carbamazepine, Stevens-Johnson syndrome (SJS), Adverse Drug Reactions (ADRs), Personalized Medicine, Toxic Epidermal Necrolysis (TEN) |
| Taxonomy: | By Niche > Genome > Genomes Data Processing By Niche > Genome > Human Genome Research |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 27 May 2024 09:26 |
| Last Modified: | 27 May 2024 09:26 |
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