A preliminary study of genetic differentiation of two important cultured oyster species, Crassostrea iredalei and C. belcheri in Malaysia based on Cytochrome Oxidase I (COI) Gene. Buletin Persatuan Genetik Malaysia, 14 (1). pp. 14-17. ISSN 1394-5750 (2009)
Abstract
This paper reports on a preliminary genetic investigation of two commercially cultured oyster species, white and black scar oysters, Crassostrea belcheri and C. iredalei, respectively. A total of 68 individuals from three different areas in Malaysia namely a C. belcheri sample from Semporna (Sabah) and two populations of C. iredalei from Trai (Sabah) and Setiu (Terengganu) were collected and analysed based on sequence analysis of cytochrome oxidase subunit I (COI). Alignment of COI gene was done using Alignment Explorer/CLUSTAL in Mega4.1. Genetic distances within and between populations were calculated using Kimura 2-parameter. Phylogenetic dendograms were generated by Neighbour-Joining (NJ) and Maximum Parsimony (MP) methods. The ingroup taxa were divided into two main clusters separating C. iredalei and C. belcheri with 99% bootstrap value. The two C. iredalei populations were homogeneous suggesting high connectivity in the South China Sea for this species. The common central haplotype in the minimum spanning networks programme is believed to be the ancestral variant for the two species. The findings from this study provides important baseline data for the aquaculture, management and monitoring of cultured populations of the oyster species
| Item Type: | Article |
|---|---|
| Keywords: | C. iredalei,, C. belcheri, Cytochrome oxidase subunit I, Genetic variation |
| Taxonomy: | By Niche > Genome > Microbial Genomes |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Rizana Mohd Radwan |
| Date Deposited: | 18 Jul 2024 02:37 |
| Last Modified: | 18 Jul 2024 02:37 |
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