Development of genetic markers for Termitomyces identification. Buletin Persatuan Genetik Malaysia, 14 (1). ISSN 1394-5750 (2009)
Abstract
Termitomyces are delicious edible mushrooms found in Africa and South-East Asia including Malaysia. These mushrooms were found to grow symbiotically with termites around termite nests. Numerous efforts have been made worldwide to develop a cultivation method for these mushrooms. Unfortunately, none of those attempts were successful. The main obstacles encountered were the difficulty to identify and isolate pure termitomyces culture. The problem became prevalent as the culture gets contaminated by other fungi. Termitomyces can easily be identified by its mushroom fruiting body eventually but certainly not at the mycelium and hyphea stages. In this study a simple PCR-based genetic marker detection method for confirmation of termitomyces at any culture stage was developed. Using this method, four distinctive PCR assays were developed using specific PCR primers designed based on the DNA sequence of the termitomyces mushroom. The PCR results showed that the PCR assays using intact termitomyces DNA as template was not suitable for this purpose. However, PCR using BamHI and EcoRI predigested termitomyces DNA as template showed identical polymorphism pattern for both termitomyces mushroom DNA and termitomyces culture DNA. Thus, the method reported here can be used for the identification and confirmation of termitomyces culture samples
| Item Type: | Article |
|---|---|
| Keywords: | Termitomyces culture, Termitomyces Genetic Markers, Cendawan busut, Cendawan daging ayam |
| Taxonomy: | By Niche > Genome > Genomes By Niche > Genome > Plant Genomes |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Rizana Mohd Radwan |
| Date Deposited: | 18 Jul 2024 03:36 |
| Last Modified: | 18 Jul 2024 03:36 |
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