Cloning and sequencing of its region of two isolates of Phellinus spp obtained from diseased roots of sentang (Azadirachta excelsa [Jack] Jacobs). Buletin Persatuan Genetik Malaysia, 11 (1): 3. pp. 5-7. ISSN 1394-5750 (2005)
Abstract
Two isolates of brown root disease fungi were obtained from diseased roots of sentang (Azadirachta excelsa). Morphological characters from macroscopic and microscopic studies suggested that both isolates were from the same genus namely Phellinus noxius and Phellinus sp. Cloning and sequencing of ITS region were conducted to investigate further the variation between the two species at molecular level. PCR-amplified ITS regions were cloned in pCR2.1 and sequenced. DNA sequences sized 723bp and 710bp were obtained for Phellinus noxius and Phellinus sp respectively. Comparison between the two sequences showed 98% similarity where three nucleotide substitutions and three insertion/deletion regions were found sized 8bp, 2bp and 3bp respectively
| Item Type: | Article |
|---|---|
| Keywords: | Phellinus, Internal Transcribed Spacer (ITS), DNA extraction, Ribosomal DNA (rDNA) |
| Taxonomy: | By Niche > Genome > Plant Genomes |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Rizana Mohd Radwan |
| Date Deposited: | 23 Jul 2024 01:04 |
| Last Modified: | 23 Jul 2024 01:04 |
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