Probiotic Properties of Bacillus Strains Isolated from Stingless Bee (Heterotrigona itama) Honey Collected across Malaysia. MDPI, 17 (278). pp. 1-15. (2019)
Abstract
This study aimed to isolate, identify, and evaluate the probiotic properties of Bacillus species from honey of the stingless bee Heterotrigona itama. Bacillus spp. were isolated from five different H. itama meliponicultures, and the isolates were characterized through Gram-staining and a catalase
test. Tolerance to acidic conditions and bile salt (0.3%), hydrophobicity, and autoaggregation tests were performed to assess the probiotic properties of the selected isolates, B. amyloliquefaciens HTI-19 and B. subtilis HTI-23. Both Bacillus isolates exhibited excellent antimicrobial activity against both
Gram-positive and Gram-negative bacteria and possessed significantly high survival rates in 0.3% bile solution for 3 h. Their survival rates in acidic conditions were also comparable to a commercial probiotic strain, Lactobacillus rhamnosus GG. Interestingly, the hydrophobicity and autoaggregation
percentage showed no significant difference from L. rhamnosus GG, a commercial probiotic strain. The
results from this study suggest that B. amyloliquefaciens HTI-19 and B. subtilis HTI-23 isolated from stingless bee honey have considerably good probiotic properties. Therefore, more studies should be done to investigate the effects of these bacteria cultures on gastrointestinal health.
| Item Type: | Article |
|---|---|
| Keywords: | stingless bee honey; probiotic Bacillus strains;, Stingless bee species |
| Taxonomy: | By Niche > Kelulut (Stingless Bee) > Anatomy By Niche > Kelulut (Stingless Bee) > Behavior By Niche > Kelulut (Stingless Bee) > Breeding By Niche > Kelulut (Stingless Bee) > Ecology By Niche > Kelulut (Stingless Bee) > Physiology By Niche > Kelulut (Stingless Bee) > Research |
| Local Content Hub: | Niche > Kelulut (Stingless Bee) |
| Depositing User: | Nur Rohaya Razali |
| Date Deposited: | 29 Aug 2024 08:42 |
| Last Modified: | 29 Aug 2024 08:42 |
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