Genome‑wide analysis of the CAD gene family reveals two bona fde CAD genes in oil palm. 3 Biotech, 12 (149). ISSN 2190-5738 (2022)
Abstract
Cinnamyl alcohol dehydrogenase (CAD) is the key enzyme for lignin biosynthesis in plants. In this study, genome-wide
analysis was performed to identify CAD genes in oil palm (Elaeis guineensis). Phylogenetic analysis was then conducted to select the bona fde EgCADs. The bona fde EgCAD genes and their respective 5′ fanking regions were cloned and analysed.Their expression profles were evaluated in various organs using RT-PCR. Seven EgCAD genes (EgCAD1-7) were identifed and divided into four phylogenetic groups. EgCAD1 and EgCAD2 display high sequence similarities with other bona fde CADs and possess all the signature motifs of the bona fde CAD. They also display similar 3D protein structures. Gene expression analysis showed that EgCAD1 was expressed most abundantly in the root tissues, while EgCAD2 was expressed constitutively in all the tissues studied. EgCAD1 possesses only one transcription start site, while EgCAD2 has fve. Interestingly, a TC microsatellite was found in the 5′ fanking region of EgCAD2. The 5′ fanking regions of EgCAD1 and EgCAD2 contain lignin-associated regulatory elements i.e. AC-elements, and other defence-related motifs, including W-box, GT-1 motif and CGTCA-motif. Altogether, these results imply that EgCAD1 and EgCAD2 are bona fde CAD involved in lignin biosynthesis during the normal development of oil palm and in response to stresses. Our fndings shed some light on the roles of the bona fde CAD genes in oil palm and pave the way for manipulating lignin content in oil palm through a genetic approach.
| Item Type: | Article |
|---|---|
| Keywords: | Cinnamyl Alcohol Dehydrogenase, CAD, Monolignols, Lignin, Oil Palm |
| Taxonomy: | By Niche > Genome > Genomes By Niche > Genome > Plant Genome Mapping |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 30 Oct 2024 01:22 |
| Last Modified: | 30 Oct 2024 01:22 |
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