Cellular and molecular profiles of anterior nervous system regeneration in Diopatra claparedii Grube, 1878 (Annelida, Polychaeta). Heliyon, 7 (2). pp. 1-11. ISSN 2405-8440 (2021)
Abstract
The polychaete Diopatra claparedii Grube, 1878 is among those organisms successfully carrying out full body
regeneration, including the whole nervous system. Thus, D. claparedii potentially can be regarded for the nervous
system regeneration (NSR) study. However, data on the property of its nervous system and the NSR profile are still
lacking. In this study, we investigated the morphology of D. claparedii anterior nervous system (ANS) and
examined the cellular and molecular profiles on its early anterior NSR. The nervous system of D. claparedii consists
of a symmetry brain with nerves branching off, circumpharyngeal connectives that connect the brain and nerve
cord as well as obvious segmental ganglia. Moreover, we identified changes in the cellular condition of the
ganglionic cells in the regenerating tissue, such as the accumulation of lysosomes and lipofuscins, elongated
mitochondria and multiple nucleoli. Furthermore, mRNA of tissues at two regenerating stages, as well as intact
tissue (non-regenerating), were sequenced with Illumina sequencer. We identified from these tissues 37,248 sequences, 18 differential expressed proteins of which upregulated were involved in NSR with noelin-like isoform
X2 turned up to be the highest being expressed. Our results highlight the cellular and molecular changes during
early phase of NSR, thus providing essential insights on regeneration within Annelida and understanding the
neurodegenerative diseas
| Item Type: | Article |
|---|---|
| Keywords: | Annelida, Nervous System, Early Regeneration, Polychaete, Ultrastructure, Transcriptome, Neurodegenerative Diseases, Micro CT Scan, Ultramicrotome, Bioinformatics |
| Taxonomy: | By Niche > Genome > Genomes |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 29 Nov 2024 09:25 |
| Last Modified: | 29 Nov 2024 09:30 |
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