Microsatellite (mEgCIR3808) Analysis in Oil Palm: An Optimisation of a Direct PCR-based using FTA Card as a Storage Medium. International Journal of Integrated Engineering, 16 (8). pp. 80-90. ISSN 2600-7916 (2024)
Abstract
This paper explores the effectiveness of Flinders Technology Associates(FTA) card, with a focus on the role of FTA card application in storing oil palm DNA for PCR analysis. The study underscores the importance of optimising the washing steps of FTA card and validation of PCR amplification of using optimised washing steps towards oil palm leaves. Therefore, the study specifically examines practices for FTA washing method for oil palm sample which is crucial and necessary in obtaining promising results. The optimised washing steps such adding centrifugation while washing the FTA cards matters and has proven to enhance the band intensity of the PCR amplification results. An optimised, proper and accurate first-basis fundamental steps will contribute the value of SSR fingerprinting as a molecular detection for Elaeisguineensis, highlighting the potential of genetic markers in oil palm research.
| Item Type: | Article |
|---|---|
| Keywords: | FTA, PCR, DNA, washing step, PCR amplification, Elaeis guineensis |
| Taxonomy: | By Niche > Genome > Agricultural Genome Mapping By Niche > Genome > Plant Genome Mapping By Niche > Genome > Plant Genomes |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 30 May 2025 02:06 |
| Last Modified: | 30 May 2025 02:06 |
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