In vitro study of Nigella sativa and thymoquinone activity on endothelial activation and monocyte adhesion. Journal of Applied Biomedicine. ISSN 1214-021X (2023)
Abstract
Introduction: Thymoquinone (TQ) is one of the bioactive compounds in Nigella sativa (NS). Also known as black seeds/cumin, it has been postulated to possess anti-atherogenic properties. However, research on the effects of NS oil (NSO) and TQ on atherogenesis remain scarce. The aim of this study is to determine gene and protein expression of Intercellular Adhesion Molecule-1 (ICAM-1), Vascular Cell
Adhesion Molecule-1 (VCAM-1), and Endothelial-eukocyte adhesion molecule (E-selectin) in Human Coronary Artery Endothelial Cells (HCAECs). Methods: HCAECs were stimulated for 24 hours (h) with 200 µg/ml of Lipopolysaccharides (LPS) and different concentrations of NSO (55, 110, 220, 440 µg/ml) or TQ (4.5, 9.0, 18.0, 36.0 µm). The effects of NSO and TQ on gene and protein expressions were measured using multiplex gene assay and ELISA assay, respectively. Rose Bengal assay was used to analyse monocyte binding activity.
Results: NSO and TQ significantly reduced ICAM-1 and VCAM-1 gene and protein expressions. TQ showed significant reduction activity of the biomarkers in dose dependent manner. HCAECs pre-treated with NSO and TQ for 24 h significantly lowered monocytes adherence compared to non-treated HCAECs.
Conclusions: NSO and TQ supplementation have anti-atherogenic properties and inhibit monocytes’ adherence to HCAECs via downregulation of ICAM-1 expression. NSO could potentially be incorporated in standard treatment regimens to prevent atherosclerosis and
its related complications.
| Item Type: | Article |
|---|---|
| Keywords: | Anti-Atherogenic, Endothelial Activation Biomarkers, Monocytes Binding Thymoquinone, Nigella Sativa |
| Taxonomy: | By Niche > Genome > Genomes By Niche > Genome > Human Genome |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 28 Aug 2025 01:49 |
| Last Modified: | 28 Aug 2025 01:49 |
| Related URLs: |
Actions (login required)
 |
View Item |