Sadiran, Siti Hajar and Sultan, Sadia and Ramasamy, Kalavathy and Faizal Webe, Jean-Frédéric Bioactive microbial metabolites from Malaysian Rainforest soil fungi. 1st ed. Asian Federation of Biotechnology, Universiti Putra Malaysia. ISBN 9789671727119 (2021)
Abstract
Microorganisms, especially soil fungi, are good sources of biologically active compounds. Twelve soil samples were collected from Sungkai (Perak), Pangkor Island (Perak), and Taman Negara (Pahang) and treated with phenol in order to select those fungal strains that would resist to that toxic compound. As a result, 91 strains were isolated and grown on a potato dextrose agar media at 28ºC. Their cultures were extracted with ethyl acetate and assayed for antimicrobial activity by disk diffusion assay against human pathogenic bacteria (Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa), and fungi (Aspergillus niger and Candida albicans). Extract SHSF (1mg/mL) showed remarkable activity with inhibition to almost all target organisms except E. coli. For example, its activity was similar to that of gentamicin (19 mm) when tested against Bacillus subtilis. The extract was then fractionated by HPLC. Twenty- five 0.5 ml-fractions were collected, dried under reduced pressure and subjected to the antimicrobial test. Three of these fractions showed significant activity in three out of five test microorganisms namely S. aureus, A. niger and C. albicans. A morphological study of the SHSF was carried out by scanning electron microscope (SEM). It is clearly appear as belonging to the genus Aspergillus. The result from internal transcribed spacer (ITS) showed that SHSF have 100% similarity with Aspergillus longivesica.
| Item Type: | Book |
|---|---|
| Keywords: | Soil Fungi, Aspergillus Longivesica, Antimicrobial Activity |
| Taxonomy: | By Niche > Genome > Genomes By Niche > Genome > Microbial Genomes |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 15 Dec 2025 06:36 |
| Last Modified: | 15 Dec 2025 06:36 |
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