Phylogenetic analysis: a more promising method to differentiate Candida haemulonii complex and its closely related species. In: Compendium of Abstracts of the 24th NIH Scientific Conference, 22 – 23 August 2023, Institute for Medical Research National Institutes of Health (NIH), Setia Alam, Selangor, Malaysia. (2023)
Abstract
Candida haemulonii complex (C. haemulonii sensu stricto, C. haemulonii var vulnera and C. duobushaemulonii),
C. auris, and C. vulturna have a close genetic relationship. As an emerging multi-drug resistant yeast species,
these Candida species are isolated from various clinical specimens of immunocompromised and neonatal
patients. Identification and differentiation of the various species within the C. haemulonii complex using omics
methods can be troublesome. Here we describe a simple phylogenetic analysis method using the discriminatory
power of the internal transcribed spacer (ITS) and large subunit (LSU) region to differentiate the Candida species
within the C. haemulonii complex. The 32 yeast isolates were identified as C. haemulonii complex, C. auris, and
C. vulturna by rRNA PCR-sequencing of the ITS and LSU region. The phylogenetic tree was then constructed
using the DNA sequences of both regions. Six distinct clades (I to VI) and five distinct clades (I to V) of C.
haemulonii complex and its closely related species were identified from both the ITS and LSU phylogenetic tree,
respectively. C. haemulonii sensu stricto and C.haemulonii var. vulnera are separated into different clades in ITS
phylogenetic tree but cluster within the same clade (Clade V) in LSU analyses. However, the analyses using ITS
sequences was prominent in differentiating C. haemulonii sensu stricto and C. haemulonii var. vulnera compared
to LSU. The outcome of this study was able to demonstrate the usefulness phylogenetic analysis using ITS and
LSU sequences to differentiate the various species of C. haemulonii complex, C. auris and C. vulturna.
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| Keywords: | Candida Haemulonii Complex, Candida Auris, Candida Vulturna, Microbial Genomes, Fungal Genomics |
| Taxonomy: | By Niche > Genome > Bacterial Genomes |
| Local Content Hub: | Niche > Genome |
| Depositing User: | Hazrul Amir Tomyang (Puncak Alam) |
| Date Deposited: | 01 Oct 2025 08:39 |
| Last Modified: | 01 Oct 2025 08:39 |
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