Genetic typing of three Malaysian Durian varieties using Simple Sequence Repeat (SSR) Markers

Genetic typing of three Malaysian Durian varieties using Simple Sequence Repeat (SSR) Markers. Malaysian Journal of Biochemistry & Molecular Biology, 26 (2). pp. 1-5. ISSN 2600-9005 (2023)



Abstract

There have been few studies on the molecular aspects of durian up to this point. Although morphological classification is practical, simple, and quick, it suffers from phenotypic plasticity due to environmental influences and age. Hence, the genetic characterization of durian varieties needs to be carried out. This study was performed to evaluate the effectiveness of three simple sequence repeat (SSR) markers to identify and discriminate three durian types: MK (Musang King), D24 and D101. Successful amplification of SSR regions were observed in the durian DNA samples. A total of 8 alleles were generated by all primers. Both DZ01 and DZ04 primers showed the highest number of polymorphic fragments. Among the three SSR primers, DZ03 is the least informative with only 2 number of alleles produced. The most important finding of this study was that each primer is unique and specific to one type of durian sample. This preliminary study showed that the SSR loci could be used as genetic markers to assist future durian breeding program.

Item Type: Article
Keywords: Polymerase Chain Reaction, Durian Types, Musang King, D24, D101, Simple Sequence Repeat Markers
Taxonomy: By Niche > Genome > Genomes
By Niche > Genome > Plant Genome Mapping
By Niche > Genome > Plant Genomes
Local Content Hub: Niche > Genome
Depositing User: Hazrul Amir Tomyang (Puncak Alam)
Date Deposited: 15 Dec 2025 06:29
Last Modified: 15 Dec 2025 06:29
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